Fig. 4

Acute platelet depletion causes dramatic changes in the clonal composition of the myeloid lineage causing the recruitment of completely new clones. A Experimental design for platelet depletion studies. B–E Pearson correlation coefficient between blood lineages (B, B cells; E, erythroid cells; M, myeloid cells; P, platelets) at 12 and 20 weeks (time points before the platelet depletion) and after platelet depletion (28 weeks). Exact r and p values (Additional file 5: Table S3). F Box plot representing clone contribution to PB before and 10 days after the platelet depletion. Statistical significance calculated using a 2-way ANOVA test with Bonferroni multiple comparison test (adjusted p values *** < 0.0001, ** < 0.001, * < 0.01, ns < 0.1). G Clonal contribution of LT-HSCs before and 10 days after platelet depletion. Heat maps representing the log fractional contributions of the top 90% most abundant contributing clones retrieved from each different bone marrow (BM) and peripheral blood (PB) cell lineage population, which were normalized per 1000. Each individual row represents the fractional contributions from an individual barcode (clone), and each individual column represents a sample. The rows are ordered by unsupervised hierarchical clustering using Euclidean distances to group barcoded clones together that manifest similar patterns of clonal contributions. The color scale on the right depicts the log fractional contribution size. Samples include Lin-Sca1 + cKit + CD150 + 48 − hematopoietic stem cells (LT-HSC), megakaryocytic progenitors (MkP), erythroid progenitors (CFU-E), platelets, erythroid cells (E), B cells (B), and CD11b + monocytes (Mac)