Fig. 2

Clonal tracking in nucleate and anucleate blood cells. A Experimental design for in vivo multilineage RNA barcoding and single-cell (BM) and bulk (PB) readouts (for 4 recipient mice in 1 experiment). Prior BM harvest mice were injected with the carrier (PBS) 10 days before the termination. These animals constitute controls for the experiment in Fig. 4. B–E Stacked bar plots representing clonal composition in PB populations (P, platelets; M, myeloid cells; E, erythroid cells; B, B cells) at 12 and 28 weeks in 4 analyzed control animals, on top depicted is the Shannon count. Based on the cumulative relative abundance of ranked barcodes (saturation curves), we focused our analysis on the top 90% of barcodes. Colors have been reused between different mice depicted in different panels—the same color represents the same barcode within the same mouse; however, between different mice (B–E), the same color represents different barcodes. F–I Pearson correlation coefficient (generated using corrplot, R package) between platelet (P), myeloid cells (M), erythroid cells (E), and B cells (B) at 12 and 28 weeks post-transplantation. Exact r and p values are included in Additional file 5: Table S3