Fig. 3

HSFA2 and HSFA3 target genes display HS-induced sustained gene expression that depends on memory HSFs. a Fraction of genes in ChIP-seq clusters that show differential expression in wild type at 4 h after ACC. Clusters c1, c5, c7, c11, and c12 are significantly enriched in genes that are upregulated at this time point (*p < 0.01, Fisher’s exact test, Additional File 2: Supplementary Data 1). b Fraction of targets genes in ChIP-seq clusters that show differential expression in wild type at 52 h after ACC. Clusters c7 and c12 are significantly enriched in genes that are upregulated at this time point (*p < 0.01, Fisher’s exact test, Additional File 2: Supplementary Data 1). c, d Median expression of genes in ChIP-seq clusters at the indicated time points and genotypes. Gene expression in clusters c7, c12, and c14 is dependent on HSFA2/HSFA3. HS-induced expression in clusters c12, c14, and to a lesser extent c7 shows sustained induction in Col-0 wild type. n, number of genes per cluster