Fig. 1

HSFA2 and HSFA3 targets during HS memory fall into 15 clusters and show differential binding strength and dynamics. a Treatment schematic for ChIP-seq and RNA-seq experiments. Four-day-old seedlings were exposed to a two-step acclimation treatment (ACC) of the indicated temperature and duration. Sampling was at the indicated time points after the end of ACC (arrowheads) or at the corresponding time point for no-HS (NHS) samples. b Median ChIP-seq signal of HSFA2 and HSFA3 in 15 cclusters at the indicated time points. CPM, counts per million; n, number of peaks in each cluster. c Memory cclusters show high binding strength at 4 h after ACC. Empirical cumulative distribution function of HSFA2- and HSFA3-bound peaks at 4 h after ACC. C7, c11, and c12 are highlighted in color. Binding of c12 is significantly higher than for all other clusters (HSFA2) and than for all other clusters except c6 (HSFA3), respectively (KS-test, p < 0.05, Additional File 2: Supplementary Data 1). Cluster c14 is not included as it contains only one peak