Fig. 5

MORC proteins influence TF binding through chromatin compaction. a Violin plot showing expression levels of genes proximal to MORC7A with Col-0, morchex mutant, Ler (wild type background for lug mutant), and lug mutant. P values were estimated by the two-sided Wilcoxon rank-sum test. b Expression levels of transcriptional factors: SEP3, PIF4, ARF6/8, TPR1, LUG, and SEU (TFs with MORC7A peaks in their promoter regions), with Col-0 and morchex mutants following heat treatment. c Transcriptomic changes of morchex mutants under normal conditions. d Transcriptomic changes of morchex mutants after 30 min of heat treatment. e TPL and LUG binding over MORC7A-unique, MORC7B-unique, MORC7-Pol V Common, and Pol V unique regions. f A representative screenshot showing increased binding of TPL and LUG on MORC7A-unique regions in the morchex mutant. g Correlation of TPL/LUG binding and ATAC-seq alterations with MORC7 binding intensity in morchex mutant. h Boxplot showing the expression levels of genes directly regulated by LUG in Col-0 and morchex mutants following heat treatment for 30 min (T30). P values were estimated by the two-sided Wilcoxon rank-sum test. i A proposed model of the RdDM-independent functions of MORC proteins