Fig. 4

Short-term caloric restriction reprograms rewires the chromatin accessibility and enhancer network in liver. A Heatmap of H3K27ac and H3K4me1 differentially enriched regions and ATAC differentially chromatin accessibility in AL and CR groups (n = 2 per group). Each row in the heatmap represents an individual differentially locus. B–D Signals of H3K27ac and H3K4me1 differentially enriched regions and ATAC differentially chromatin accessibility in AL and CR groups (n = 2 per group). E,F Differentially representative enriched region of open chromatin, H3K27ac, H3K4me1, and gene expression levels in AL and CR groups (n = 2 per group). E Depicts the Cyp7b1 locus which has lower chromatin accessibility, acetylation, and transcript levels in CR group. F Depicts the Fmo3 locus which has higher acetylation, methylation, and transcript levels in CR group. G Distance to TSS of gain or loss H3K27ac regions between AL and CR groups (n = 2 per group). H Genomic features of the location of H3K27ac CR gain and loss peaks in AL or CR group (n = 2 per group). I Motif analysis of differentially H3K27ac enriched regions in AL or CR group (n = 2 per group). J Motif analysis of differentially open chromatin regions in AL or CR group (n = 2 per group). K Venn diagram of the differentially enriched H3K27ac regions and differentially open chromatin regions (n = 2 per group). L Motif analysis of differentially enriched H3K27ac regions and differentially open chromatin regions in AL or CR group (n = 2 per group). M,N GO pathway analysis of loci near CR gain or loss H3K27ac regions. O Gene expression in CR gain or loss H3K27ac regions (n = 2 per group). Significance was calculated using non-paired two-tailed Student’s t test. ∗ p < 0.05, ∗  ∗ p < 0.01, ∗  ∗  ∗ p < 0.001, ∗  ∗  ∗  ∗ p < 0.0001