Fig. 1
From: A comprehensive map of human glucokinase variant activity
Yeast complementation as a readout for human glucokinase variant activity. A Yeast growth assay of wild-type (WT) and hxk1Δ hxk2Δ glk1Δ yeast strains on galactose and glucose media. B The growth of the hxk1Δ hxk2Δ glk1Δ yeast strain expressing either a vector control, wild-type GCK (WT) or a GCK variant was compared on media containing galactose or varying concentrations of glucose. C Growth assay of different combinations of vectors (-), GCK and GKRP expressed in the hxk1Δ hxk2Δ glk1Δ yeast strain on galactose and glucose media. D Illustration of the multiplexed assay for GCK variant activity