Fig. 7

Inhibiting p300 reduces the lactylation of YY1 and suppresses angiogenesis in OIR. a p300, YY1, and YY1-Kla in the retinas of normal control (Normal P17) and OIR (OIR P17) mice (n = 3 per group). b Representative images of YY1-Kla co-stained with microglia (Iba1) in retina of OIR mice (OIR P17) and control mice (Normal P17), with quantification of YY1-Kla intensity (n = the number of Iba1⁺ cells); scale bar, 20 μm. c Quantification of YY1-Kla and FGF2 in the retinas of OIR mice (OIR P17) treated with A-485 (200 μM, 1 μl/eye) and DMSO (0.1%) were analyzed by Western blotting (n = 3 per group). d Representative images of YY1-Kla co-stained with microglia (Iba1) in retina of OIR mice treated with A-485 (200 μM, 1 μl/eye) and OIR mice treated with DMSO (0.1%), with quantification of YY1-Kla intensity (n = the number of Iba1⁺ cells); scale bar, 20 μm. e Confocal images of CD31-stained retinal flat mounts in OIR P17 mice treated with A-485 (200 μM, 1 μl/eye) and DMSO (0.1%). The relative proportion of neovascularization in the retina was calculated and measured by ImageJ (n = 4 mice per group); scale bar, 1000 μm. f Representative images of FGF2 co-stained with microglia (Iba1) in retina of OIR mice (OIR P17) treated with A-485 (200 μM, 1 μl/eye) and DMSO (0.1%), with quantification of FGF2 intensity (n = the number of Iba1⁺ cells); scale bar, 20 μm. g Confocal images of CD31 and Iba1-stained retinal flat mounts in OIR P17 mice treated with A-485 (200 μM, 1 μl/eye) and DMSO (0.1%); the yellow arrow denotes the resting-state of microglia, and the green arrow denotes the activated state of microglia; scale bar, 50 μm. *p < 0.05; ***p < 0.001