Fig. 6

p300 affects the lactylation of YY1 and then regulates angiogenesis in vitro. a Quantification of p300 in the HMC3 cells under normoxia or hypoxia for 24 h (n = 3 per group). b Co-IP analysis of YY1 and p300. c Colocalization of YY1 and p300 by double-label immunofluorescence. d The Pan-Kla level in the HMC3 cells overexpressing p300 (n = 3 per group). e Overexpression of p300 increased YY1 lactylation. Lactylation of YY1 in control group, or oep300-WT group was detected by YY1-Kla antibodies (n = 3 per group). f The expression of FGF2 and p300 in the HMC3 cells overexpressing p300 (n = 3 per group). g Quantification of FGF2 and Pan-Kla in the HMC3 cells treated with 0, 5, 10, and 20 μM A-485 under hypoxia for 24 h (n = 3 per group). h Applying A-485 decreased YY1 lactylation. Lactylation of YY1 with 0 μM A-485, or 20 μM A-485 was detected by YY1-Kla antibodies (n = 3 per group). *p < 0.05; **p < 0.01; ***p < 0.001