Fig. 5

YY1 lactylation contributes to angiogenesis by regulating FGF2 expression. a The mRNA expression of VEGFA, FGF2, MMP2, MMP9, and ANGPTL6 in the retinal tissue of normal control (Normal), OIR (DMSO), and OIR (PLX3397) mice (n = 3 per group). b The mRNA expression of VEGFA, FGF2, MMP2, MMP9, and ANGPTL6 in HMC3 cells in the three groups subjected to hypoxia for 0, 12, and 24 h (n = 3 per group). c The protein expression of FGF2 in the retinas of normal control (Normal), OIR (DMSO), and OIR (PLX3397) mice (n = 3 per group). d The protein expression of FGF2 in the HMC3 cells subjected to hypoxia for 0 and 24 h (n = 3 per group). e Representative images of FGF2 co-stained with microglia (Iba1) in retina of OIR mice (OIR P17) and control mice (Normal P17), with quantification of FGF2 intensity (n = the number of Iba1⁺ cells); scale bar, 20 μm. f The protein expression of FGF2 in HMC3 cells exposed to hypoxia 24 h (Hypoxia Control), hypoxia for 24 h + YY1 WT transfection (Hypoxia + WT), and hypoxia for 24 h + YY1 K183R transfection (Hypoxia + K183R) (n = 3 per group). g ChIP-qPCR analysis of the indicated promoters was performed by using of YY1 antibody in HMC3 cells treated with normoxia and hypoxia (n = 3 per group). h ChIP-qPCR analysis of the indicated promoters was performed by using of YY1 antibody in HMC3 cells overexpressing WT and K183R YY1 (n = 3 per group). i The luciferase activity of the FGF2 promoter driven reporter vector was measured in response to YY1 WT or K183R cotransfection under hypoxia (n = 6 per group). NS, non-significance; *p <0.05; **p < 0.01; ***p < 0.001