Fig. 1

Detection of FMT donor genomes in FMT recipients and publicly available gut metagenomes. In both heat maps, each column represents a donor genome, each row represents a metagenome, and each data point represents the detection of a given genome in a given metagenome. Purple rows represent donor metagenomes from stool samples collected over 636 days for A donor A and 532 days for B donor B. Orange rows represent recipient pre-FMT metagenomes, and blue rows represent recipient post-FMT metagenomes. Rows are arranged in descending chronological order with respect to each subject. The intensity of purple, orange, and blue color scales represents the detection value for each genome in each metagenome, with a minimum detection of 0.25. Genome columns are clustered according to their presence or absence in all metagenomes (Euclidean distance and Ward clustering). The three columns to the right of the heatmaps display, for each metagenome row: (X) the number of metagenomic short reads in millions, (Y) the percent of metagenomic short reads recruited by genomes, and (Z) the taxonomic composition of metagenomes (based on metagenomic short reads) at the phylum level. The first row below each heat map (Q) provides the phylum-level taxonomy for each donor genome. Finally, the 11 bottommost rows under each heat map show the fraction of healthy adult metagenomes from 11 different countries in which a given donor genome is detected (if a genome is detected in every individual from a country it is represented with a full bar and a value of 1). The dendrograms on the right-hand side of the country layers organize countries based on the detection patterns of genomes (Euclidean distance and Ward clustering). Purple and red shaded countries represent the two main clusters that emerge from this analysis, where purple layers are industrialized countries in which donor genomes are highly prevalent and red layers are less industrialized countries where the prevalence of donor genomes is low. A maximum resolution version of this figure is also available at https://doi.org/10.6084/m9.figshare.15138720 [26]