Fig. 3

Experimental validation of TrEMOLO specificity of TE insertion/deletion detection. a PCR performed using long DNA molecules extracted from the G0-F100 (control) and G73 Drosophila lines (genomic DNA extracted using Short-Read Eliminator kit: G73-SRE sample). The name of the TE family identified by TrEMOLO is indicated followed by the chromosome arm and the genomic coordinate in the G73 (183×) assembly. The frequencies determined in the G73 (depth 183×) of each TE insertion tested by PCR are indicated as percentages in blue. b Illustration of the primers used to detect a hobo excision. c PCR performed using long DNA molecules extracted from the G0-F100 (control) and G73 Drosophila lines. The primer sequences and amplicon expected sizes are in Additional file 1: Table S1