Fig. 6

NSD2 mutant patient samples reflect B-ALL cell line data. a PCA of 3 matched diagnosis (NSD2-Low) and relapse (NSD2-High) patient pair gene expression data. b Heatmap of significantly expressed genes upon relapse (from NSD2 Low to NSD2 High: abs(L2FC) > 0.32, p-adjusted < 0.05). c Volcano plot demonstrating differentially upregulated genes highlighted in red and downregulated genes in blue(abs(L2FC) > 0.32, p-adjusted < 0.05). d Venn diagrams demonstrating significant overlap of patient NSD2-High upregulated genes and cell line NSD2-High upregulated genes with BA compartment switches and insignificant overlap of patient NSD2-High downregulated genes and cell line NSD2-High upregulated genes with BA compartment switches (one-tailed Fisher’s exact test). e Barplot demonstrating number of patient genes overlapping cell line compartment switches and shifts colored by cell line. f Correlation boxplot showing patient gene expression changes within cell line compartment changes (NSD2-High/NSD2-Low). g KEGG 2021 pathway enrichment analysis of patient genes upregulated in NSD2-High patients (abs(L2FC) > 0.32, p-adjusted < 0.05) that overlap with cell line B to A compartment switches, B to less B, and A to more A compartment shifts from any of the three cell lines. Starred pathways represent pathways previously shown in NSD2 models