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Fig. 6 | Genome Biology

Fig. 6

From: Loss of MLL3/4 decouples enhancer H3K4 monomethylation, H3K27 acetylation, and gene activation during embryonic stem cell differentiation

Fig. 6

Gene-centric analysis reveals a subset of distal loci associate with MLL3/4-dependent formative genes. A Pie charts showing fraction of normally gained H3K4me1, H3K27ac, or gene expression that are dependent on MLL3/4 or not. Two hundred fifteen formative genes represent those of the 283 formative down genes from Fig. 1K without preexisting naive expression defects (Log2CPM naive DKO/WT > −1). B Clusterprofile analysis of Biological Processes Gene Ontology for 215 MLL3/4 dependent formative genes. C Fold change Log2CPM of H3K27ac density for H3K4me1/H3K27ac+ enhancers associated with formative genes that gained expression during transition in an either MLL3/4 independent or dependent fashion. Only 131 out of 215 dependent genes and 820 out of 1387 independent genes were able to be associated with an enhancer. D Genome tracks for known and predicted formative targets of MLL3/4 Lefty1, Brachyury/T, and Fgf8. E Model for the role of MLL3/4-independent and MLL3/4-dependent mechanisms in the regulation of gained gene expression during the naive to formative transition

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Genome Biology

ISSN: 1474-760X

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