Fig. 4.

H3.3 facilitates active RNAPII engagement at promoters. A Box plot showing GRO-seq signal at promoters of expressed genes (TSS − 30 bp to TSS + 250 bp) in WT and H3.3 KO ESCs. The bottom and top of the boxes correspond to the 25th and 75th percentiles, and the internal band is the 50th percentile (median). The plot whiskers correspond to 1.5× interquartile range and outliers are excluded. p-values determined by Wilcoxon rank sum two-side test. B Scatterplot showing differential binding scores of investigated TF motifs and median ratio (log2) of GRO-seq signal at promoters containing each motif. Each TF is represented by a single dot (n = 395). Representative TF motifs are labeled in green. Dashed red line represents a linear fit