Fig. 6

Histopathology of DIC-F1 pig with metastatic pancreatic adenocarcinoma. a–b H&E and IHC staining of sections from the different pancreatic head and tumor sites. CK19, a ductal origin marker. PCNA, a proliferative marker. Vimentin, a mesenchymal marker. E-cadherin, an epithelial lineage marker. pERK, a KRAS activation effector. Scale bars, 100μm. c Representative immunofluorescence staining results of different PDAC regions. CK19-positive cells were also PCNA positive. CK19 was positive at the membrane of tumor cells, whereas PCNA was positive at the nucleus of tumor cells. αSMA, pancreatic stellate cell marker. Scale bars, 200 μm. d H&E staining of other metastatic organs (liver, intestine, diaphragm, and lymph node) in PDAC pig. Scale bars, 1000 μm or 100 μm as indicated. e–h IHC staining sections from the liver (e), intestine (f), diaphragm (g), and lymph node (h) to analyze the expression of PCNA, CK19, and pERK, respectively. Scale bars, 1000 μm or 100 μm as indicated