Fig. 4

Pro- and anti-inflammatory macrophages are negatively correlated and highly diverse between gastric cancer patients. A UMAP of 10,646 myeloid cells color coded for tissue (bottom) and cluster annotation (top). B Bar plot of a proinflammatory (IL1B, CCL20, S100A8, S100A9) and anti-inflammatory (CD163, MAF, SEPP1, APOE) gene score per cell cluster (top). Dotplot of top markers per myeloid cluster showing the scaled average expression and the percentage of expression (bottom). C Log ratio of the average fraction per myeloid cluster annotation in tumor to normal tissue (n = 20). Wilcoxon rank-sum test with Holm correction, *: p < 0.05, **: p < 0.01, ***: p < 0.001. D Bar plot of the proinflammatory and anti-inflammatory gene signature from B in scRNA-seq data of our cohort (n = 20) and the TCGA-STAD cohort (n = 407). E Overall survival of gastric cancer patients in TCGA, groups split by expression level of the proinflammatory (left) and anti-inflammatory (right) gene signatures from B