Fig. 2

Transcriptional reprogramming in cancer-associated fibroblasts. A UMAP of 16,492 fibroblast cells color coded for tissue (top) and cluster annotation (bottom). B Log ratio of average fraction per fibroblast cluster in tumor to normal tissue (n = 20) (top). Wilcoxon rank-sum test with holm correction, *: p < 0.05, **: p < 0.01, ***: p < 0.001. Dotplot showing the scaled average expression and the percentage of expression of top markers per fibroblast cluster (bottom). C Scaled average expression of fibroblast implicated gene clusters (Additional file 1: Fig. S4). D Top marker genes for F12-ANGPT2 and F13-CTHRC1 connected to gene cluster U6 and U7 respectively. E Overall survival of gastric cancer patients in TCGA, groups split by the top 20 marker gene signature of F13-CTHRC1. Gene signatures U1, U6, and U7 reflect gene clusters that were upregulated in gastric tumor samples while gene signatures D9 and D11 reflect gene clusters that were downregulated in gastric tumor samples