Fig. 3

Identifying functional sets of proteins that covary with CDC markers. a Distributions of DNA content for FACS-sorted cells used to identify proteins whose abundance varies with CDC phase. b Correlations between CDC protein markers computed within the single cells from each type in the pSCoPE dataset. c PCA of melanoma and monocyte cells in the space of CDC periodic genes. Cells in each PCA plot are colored by the mean abundance of proteins annotated to the indicated phase. d The boxplots display distributions for correlations between the CDC phase markers and proteins from the RNA catabolism protein set. The correlations were computed for single cells randomly partitioned into two non-overlapping subsets (marked by 1 and 2) to explore their reproducibility. The difference between the distributions for a protein set was evaluated by 1-way ANOVA analysis to estimate statistical significance. The distributions for other protein sets that significantly (FDR < 5%) covary in a similar way between the two cell lines are summarized with their medians plotted as a heatmap. e Similar analysis and display as in panel d was used to visualize protein sets whose covariation with the CDC differs between the two cell types