Fig. 3
From: INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencing
INSERTseq reveals integration patterns in rAAV-8 and PB genome editing frameworks. analysis reveals rAAV-8 integration hotspots in mice. a AAV-8 CMV GFP was administered to mice using retro-orbital injection, and liver tissue was extracted for INSERTseq analysis 1 week upon administration. b Genome-wide map displaying integrations of rAAV in two replicate samples. The Venn diagram summarises the overlap of common insertions of both replicates. c Features at AAVs integration sites. d Heatmap of the area under the curve (AUC) representing the association of integration of AAV mouse liver samples (replicate 1 and 2) with different features. An AUC value higher than 0.5 indicates positive association (more than random) with the feature, whilst an AUC lower than 0.5 indicates negative association. e Genome wide integration of a Cas9-HyPB transposase, representing off-target integration (red) and on-target integration (green) at the TRAC gene (ch:r1422,547,659). Fraction of on-target (0.016) and off-target (0.98) insertions is displayed in a stacked barplot. f Dotplot displays on-target integration events in both + strand (blue dots) and − strand (red dots). Vertical lines represent region targeted by gRNA (outter black lines) and Cas9 cutting site (middle black line)