Fig. 5

Ectopic expression of ZCWPW1 promotes DSB repair in human somatic cells. a After exposure to ETO for 3 h, the expression levels of γH2AX were measured by western blot in control and ZCWPW1-overexpressing HeLa cells. b Neutral comet assay to evaluate the extent of DSB repair in HEK293T cells treated with hydroxyurea (HU) for 16 h after transfection with pCAG or ZCWPW1-EGFP-pCAG for 24h. Scale bar, 20μm. c Bar plot showing the ratio of tail DNA in the neutral comet assays. ****P < 0.0001 by two-tailed unpaired Student’s t test. Data are representative of six independent experiments. d Schematic illustration of the GFP-based homologous recombination reporter assay. e Expression of ZCWPW1 promotes homologous recombination. U2OS DR-GFP cells expressing ZCWPW1 were electroporated with a pCBASce construct and were assayed for homologous recombination efficiency by monitoring GFP levels at 48 h post electroporation. **P < 0.01 by Student’s t test. Data represent the mean ± SEM from three independent experiments