Fig. 1

Dyskerin regulates the recruitment to polysomes of snoRTs. A Representative Western blot analysis image (top) and densitometric analysis of 5 independent replicates (bottom) of dyskerin levels after DKC1 mRNA KD in MCF7 cells. Data are shown as means + SD. Paired Student’s t tests were performed relative to controls. ****p < 0.001. B Representative polysome profiles obtained by 15–50% sucrose density gradient centrifugation from control (black) and DKC1 KD (red) MCF7 cells. Three independent replicates were used. Ten percent of each fraction was pooled to reconstitute total mRNA, while the remaining polysomal fractions were pooled together. Reverse transcribed polysomal and total mRNAs were sequenced using a next-generation sequencing (RNA-seq) approach with a depth of about 50–60 M usable reads. Processed data were analyzed for significant differences in mRNA levels normalized signals between polysomal and total RNA fractions. C Count-based differential expression analysis of total cytoplasmic (left panel) and polysomal-recruited (right panel) transcripts. Differentially expressed transcripts are depicted as red dots, while transcripts gene names of interest are indicated