Fig. 3
From: PtoNF-YC9-SRMT-PtoRD26 module regulates the high saline tolerance of a triploid poplar
PtoRD26 directly and positively regulates SRMT expression. A The constructs of the effector and reporter in the dual-luciferase assay. The pCXSN empty vector contains a ccdB gene that is a negative selection marker to eliminate the self-ligation background after transformation. BPtoRD26.1 enhanced fluorescence intensity of LUC driven by the SRMT.3 promoter (SRMT.3pro) compared to the control. The mean ± s.d. of three biological replicates is shown. Asterisks indicate statistically significant differences (two-sided Student’s t test, **P < 0.01). C The distribution of the PtoRD26 binding element (RE1 and RE2) in the SRMT.3 promoter. D EMSA indicating that PtoRD26.1 can bind competitively by cold probes to RE2, but not to RE1. E The Y1H assay verifying the binding of PtoRD26.1 to RE2 in the SRMT.3pro