Fig. 6

OsFBK16 is the hub interactor of OsPALs and OsPAL6 confers rice blast resistance. a Confirmation of the interaction between OsPAL family members and OsFBK16 in yeast. DDO, SD-Leu-Trp, QDO, and SD-Leu-Trp-His-Ade. b Colony number of OsFBK6 screened by OsPAL1-7 from the rice UbE3 library. c Interaction between OsPAL family members and E3 ligase OsFBK16. d Degradation assay of OsPAL5 and OsPAL6 by OsFBK16 in vivo. N. benthamiana leaves were agro-infiltrated with OsPAL5-GFP or OsPAL6-GFP and OsFBK16-HA or GUS-HA. Samples were collected, and total protein was extracted. The protein abundance of OsPAL5 and OsPAL6 were detected by immunoblotting using an anti-GFP antibody, and OsFBK16 and GUS were detected using an anti-HA antibody. OsPAL5 and OsPAL6 transcript levels were measured by RT-PCR, and ACTIN was used as the internal control. e–g Disease symptoms of two representative OsPAL6-GFP overexpression lines and NPB seedlings after punch inoculation with RB22. e The images were taken 14 days after inoculation. f The disease lesion area was measured with the ImageJ software. An asterisk indicates a significant difference between WT and OsPAL6-GFP overexpression plants according to Student’s t test (**p < 0.01). g Relative fungal biomass was calculated by measuring the expression of the M. oryzae MoPot2 with the DNA-based quantitative PCR assay. An asterisk indicates a significant difference between WT and OsPAL6-GFP overexpression plants according to Student’s t test (**p < 0.01)