Fig. 7

GoPeaks has higher specificity and sensitivity for H3K27ac CUT&Tag peaks with broad and narrow peak shapes. a ROC curves quantifying the recall and false positive rates and b PR curves quantifying the precision and recall rates of H3K27ac CUT&Tag data from H3K27ac ChIP-seq data. Both ChIP-seq and CUT&Tag datasets were generated in Kasumi-1 cells. Colors indicate the peak calling method. c Number of high-confidence peaks identified from H3K4me3 CUT&Tag data per peak calling method. d Overlap of high-confidence peaks identified by each peak caller. e Percent of total H3K27ac ChIP-seq standard peaks that are identified by each peak caller. f Example peaks near at the TET1 gene. IgG replicates are the negative controls. Consensus peak calls for each method are shown. Tracks are CPM normalized and are scaled to the range [0–4.26] by IGV. Tracks are depicted on the GRCh38 genome assembly