Fig. 4

Bookend performance on single mouse cells. A Reconstruction of Spike-In RNA Variants (SIRVs) from 96 paired-end 100 bp SMARTer libraries of single mESCs. Each vertical bar depicts the assemblies from one cell, ordered from highest (bottom) to lowest (top) estimated abundance. Colored boxes match a true isoform of the given input concentration; gray boxes are false assemblies. B SIRV assembly performance as a function of increasing sequencing depth. F-measure (right) is the harmonic mean of sensitivity and precision. C Boxplots showing percent validation of 5′ ends with SLIC-CAGE support within the given windows for 96 single mESC assemblies. D Boxplots as in (C) showing 3′ end validation by 3P-Seq peaks. E Percent of intergenic assemblies (no overlap with RefSeq) in single cells which have ≥1 matching Capture Long-Seq read from the mouse CLS atlas