Fig. 2

NT-seq detects both adenine and cytosine methylation in oligonucleotides. a The inverse of A to G ratio at adenine sites between unmodified control and 6mA modified oligo. b Linear regression between A to G frequency at 6mA site and the percentage of 6mA modified oligo. c The inverse of C to T ratio at cytosine sites between unmodified oligo and oligo modified by BamHI methyltransferase. d Linear regression between C to T frequency at 4mC position 39 and the percentage of 4mC modified oligo. e Linear regression between C to T frequency at 4mC position 42 and the percentage of 4mC modified oligo. f C to T ratio at cytosine sites between unmodified control and 5mC modified oligo. Modified adenine or cytosine sites are labeled in red. Adenine or cytosine sites inside the primer regions are not included. Dots represent the mean and error bars represent standard deviation. All samples were replicated three times. One replicate for 25%, 50%, and 100% of 4mC modified oligo samples was not used due to library prep and sequencing depth issues