Fig. 1
From: Strand asymmetry influences mismatch resolution during single-strand annealing
Experimental approach. a Mouse ES cells in culture are co-transfected with a barcoded reporter library and with the Sleeping Beauty 100X transposase. b The reporters in the pT2 transposon backbone are integrated at random in the mouse genome. c Mismatches occur during the repair of a double-strand break induced by the transient expression of I-SceI. If the double-strand break is repaired through non-homologous end joining (NHEJ), no mismatch is formed. If it is repaired by single-strand annealing (SSA), a mismatch is formed and repaired in favor of one of the two nucleotides. Sequencing the construct reveals the outcome of DNA repair at different locations identified through the barcode