Fig. 6

MISTy signatures can uncover clinically relevant features in IMC data from 415 breast cancer samples. A Change of the total number of estimated important interactions per grade (Importance ≥ 0.5). B Changes in the tumor microenvironment can be visualized by network community plots representing the juxtaview for tumor grades 1 and 3. For example, the green cluster represents a constant link in the juxtaview between luminal- (CK8/18+) and basal-like (CK5+) cell types across all tumor grades, while the yellow cell cluster shows an increased interaction with tumor progression of immune cells (CD68+/CD45+), B cells (CD20+) and T cells (CD3+) with cells potentially undergoing EMT (Twist+). C Importance signatures visualized as the first two components of a PCA highlight the separation of grade and D clinical subtype. Kaplan-Meier curves and p-values of a log rank test based on stratification by estimated importance of MISTy predictor-target interactions that were found to be correlated with the patient outcome: E CC3.cPARP and EGFR in the intraview; F SMA and pHH3 in the juxtaview, and G Vimentin and EGFR in the paraview