Fig. 4
From: Multi-omics analysis dissects the genetic architecture of seed coat content in Brassica napus
Functional characterization of BnaTT8 as a positive regulator of seed lignin biosynthesis in B. napus. a Seeds of CRISPR/Cas9-induced BnaA09.TT8 and BnaC09.TT8 homozygous double mutant lines (L14, L17, L21, L18, L24, and L30). Bar = 1 cm. b the seed coats of L21 and L24. Bar = 1 cm. Determination of thickness of seed coat (c), SCC (d), lignin content (e), and seed oil content (f) in the BnaTT8 double knockout mutant seeds and the control (WT). Values are means ± SD of three biological replicates (n = 6). Student’s t-test was used for statistical analysis between the BnaTT8-sgRNA lines and WT (*, P < 0.05)