Fig. 2

CUT&Tag2for1 simultaneously profiles active and repressive elements. a Schematic describing the CUT&Tag2for1 rationale: a joint CUTAC-CUT&Tag profile is generated using antibodies against Pol2S5p and H3K27me3, and enriched regions are assigned post-hoc based on fragment size. b Genome browser screenshot showing a CUT&Tag2for1 profile (green) in comparison with H3K27me3 CUT&Tag (red) and Pol2S5p-CUTAC (blue) for a representative region in K562 cells, along with enriched peaks. c CUT&Tag2for1 large (red) and small (blue) fragment peaks as assigned by fragment size are shown. d Heatmaps describing H3K27me3 CUT&Tag (red) and RNA Pol2S5p-CUTAC (blue) enrichment at large fragment (left) or small fragment (right) peaks as defined from CUT&Tag2for1 profiles