Fig. 3

ABE-induced DNA mutations in different T-DNA insertion events. a IGV browser views showing the read coverages at T-DNA insertion sites. Lines 46bM_s2 and 46bM_s3, 49bM_s2 and 49bM_s3, and 49bAG_s3 and 49bAG_s4 were germinated from the same calli. Regions in red rectangles are the T-DNA insertion sites. b Number of SNVs and A>G SNVs, and percentage of A>G SNVs. Set 1 represents the unique SNVs only in 46bM_s2, 49bM_s2, and 49bAG_s3. Set 2 represents the unique SNVs only in 46bM_s3, 49bM_s3, and 49bAG_s4. Overlap represents the overlapping SNVs in 46bM_s2 and 46bM_s3, 49bM_s2 and 49bM_s3, and 49bAG_s3 and 49bAG_s4. c Number of SNVs and A>G SNVs, and percentage of A>G SNVs in plants with partial or whole T-DNA insertions of rBE50 or rBE53. Each bar represents the mean value, each error bar represents the standard error, and each dot represents the number of SNVs, the number of A>G SNVs, and percentage of A>G SNVs of each plant. (ns) denotes p-value > 0.1, (*) denotes p-value < 0.1 (one-tailed Wilcoxon test)