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Fig. 2 | Genome Biology

Fig. 2

From: FIONA1 is an RNA N6-methyladenosine methyltransferase affecting Arabidopsis photomorphogenesis and flowering

Fig. 2

In vitro methylation activity of FIONA1. a-c Representative LC-MS/MS chromatograms of d3-m6A formed in RNA probes treated with or without FIONA1. The in vitro RNA N6-adenosine methylation activities of FIONA1 were tested using different RNA probes (numbered 1–6) containing sequence of UACAGAGAA, GGACC and UGUAU in a linear or looped structure in the presence of isotope-labeled cofactor d3-SAM. d The peak area ratio of d3-m6A versus one G in the probe (as internal control) was used to evaluate the methylation activities of FIONA1 towards different RNA oligos. Data are means ± SD for 2 biological replicates × 2 technical replicates

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Genome Biology

ISSN: 1474-760X

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