Fig. 1

Presence of 4acC in Arabidopsis gDNA. a Immuno-southern blot of 4acC. A specific anti-4acC antibody was used to detect 4acC signals in gDNA extracted from 3-week-old Col-0 WT plants. The amount of gDNA loaded is indicated. b Dot blot showing the effect of hydroxylamine (100 mM, pH 7.0, 65 °C, 2 h) on 4acC in cellular gDNA. The amount of gDNA loaded is indicated. c−e UPLC-ESI-MS/MS analysis of 4acC in the 4acC standard (c), Arabidopsis gDNA (d), and Arabidopsis gDNA treated with NH₂OH samples (e) with mass transitions of m/z 270.13 to 154.08 and m/z 270.13 to 112.03 showing peaks at a retention time of approximately 2.81 min. f UPLC-ESI-MS/MS quantification of 4acC levels in Arabidopsis gDNA samples without or with NH₂OH treatment. The error bars denote the means ± SDs, n = 3 biological replicates