Fig. 3

CMV 2b binds endogenous sRNAs. A Representative pictures of 2b-GFP overexpression transgenic lines. B sRNA categorization in input and 2b-IPed sRNA libraries. C Proportion of different size classes of mRNA-derived sRNAs in input and 2b-IPed samples. D Scatterplot of sRNA accumulation (log10 of RPMs) for individual miRNA families. Elements that accumulate 2-fold or higher sRNAs in 2b-IP samples are highlighted in blue while elements that accumulate 2-fold or higher in input samples are highlighted in red. E Violin plot of RNA expression (log10 RPKM) for mRNAs that accumulate sRNAs more than 2-fold in 2b IPed samples (left panel), more than 2-fold in input samples (center panel), and the rest of mRNAs (right panel). p values are indicated in the comparisons and indicate the result of a paired t test with 2 tails. F Size distribution of the vsiRNAs loaded into 2b or input samples. G Proportion of 5′ nucleotide distribution in vsiRNAs loaded in 2b or input samples. Two bioreplicates from 2b-IPed sRNA libraries were generated and analyzed