Fig. 3

ATAC-seq analysis of genome-wide chromatin accessibility upon neural transdifferentiation of pericytes. a Heatmaps show density of mapped ATAC-seq reads 2 kb up- and downstream of ENSEMBL-annotated transcriptional start sites (TSS) in GRCh38 (hg38). For every time point (n = 6 time points), data were partitioned into 5 clusters based on k-means algorithm. b Graphs summarize GO enrichment analysis of ATAC-seq profiles of pericytes upon transdifferentiation (Analysis platform: Metascape). The top graph shows functional clustering of genomic loci that become inaccessible soon after induction of transdifferentiation, at t = 2 min. The middle and bottom graphs show inaccessible genomic loci in pericytes that become accessible at t = 2 and 4 min after induction of transdifferentiation. c ATAC-seq peaks demonstrate accessibility of genomic loci encoding MYOD1 and MASH1 loci at baseline and at t = 2 and 4 min post-induction of transdifferentiation