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Fig. 3 | Genome Biology

Fig. 3

From: Wheat in vivo RNA structure landscape reveals a prevalent role of RNA structure in modulating translational subgenome expression asymmetry

Fig. 3

Translation efficiency (TE) correlates with in vivo RNA structures in tetraploid Kronos. a Line plot showing SHAPE reactivities of highly and lowly translated transcripts, as shown by 10% of highest or lowest TEs. SHAPE reactivities of different genic regions across highly (red) or lowly translated (bluish green) mRNAs were averaged. mRNAs were aligned by their translation start and stop codons. The inset shows strong 3nt periodicity of SHAPE reactivity in the CDS region, but not in the 5′UTR or 3′UTR of highly translated mRNAs, nor in the 5′UTR, CDS, or 3′UTR of lowly translated mRNAs. b–d Relationship between RNA structure and TE across different genic regions. The scatter plots showed the correlation between base-pairing probability and TE across 5′UTR (b), CDS (c), and 3′UTR (d)

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