Fig. 1

Translational landscape in tetraploid Kronos. a Representative chromatogram of Kronos lysates separated by sucrose gradient. The peaks representing 80S monosome and polysome are indicated. b–d Correlations between translation efficiency (TE) and RNA abundance, GC content, and tRNA adaptation index (tAI) in Kronos. The correlation coefficients between translation efficiency and RNA abundance, GC content, and tAI are 0.21, 0.54, and 0.07, respectively (P values < 10^-16). e Scatter plot showing the TE differences between A and B subgenomes. The A subgenome homoeologs with significantly higher or lower TE values than that those in the B subgenome, or without significant differences between A and B subgenomes are coloured with blue, red, or gray, respectively (P < 0.05, by Student’s t test followed by Benjamini-Hochberg correction, NSD, no significant difference). f Translational patterns between the A and B subgenomes of different biological functions. Each listed item includes at least ten homoeologous pairs. The error bar indicates the standard error in TE differences between the homoeologs of the A and B subgenomes