Fig. 2
From: SCEPTRE improves calibration and sensitivity in single-cell CRISPR screen analysis
SCEPTRE: Analysis of single-cell perturbation screens via conditional resampling. A schematic and outline of the SCEPTRE methodology for one gene and one gRNA. SCEPTRE estimates the probability of gRNA detection in each cell based on its technical factors. It then builds a null distribution for the negative binomial z-value by independently resampling gRNA presence or absence for each cell according to these probabilities to form “negative control” datasets. A skew-t distribution is fit to the resulting histogram to obtain precise p-values based on a limited number of resamples, against which the original NB z-value is compared. The dashed line shows the standard normal distribution, against which the NB z-value typically would be compared