Fig. 5

In vivo validation of predicted heart-specific mammalian candidate genes in Drosophila. A Drosophila melanogaster orthologs of candidate Mus musculus cardiac genes and their known expression in cardiac tissue. ^*Obtained from DIOPT (https://www.flyrnai.org/cgi-bin/DRSC_orthologs.pl, [14]). ^#Data from [25] where + denotes low, ++ medium, +++ high, ++++ very high heart expression, and “P” denotes gene product detected via proteomics from [15]. n.d. not detected. n.t. not tested. ^‡Mortality index (MI) is calculated as the number of curly minus straight-winged progeny / curly progeny × 100 from crosses between 4 × Hand-Gal4/CyO and the UAS-RNAi lines. Only crosses that produced MIs over 10% (larger deviation than control crosses) are shown. B The relative proportions of phenotypic severity classes from RNAi knockdown, where blue is unaffected (normal, MI < 10%), green is low (MI = 11–30%), yellow is medium (MI = 31–60%) and red is severe (MI > 61%)