Fig. 4

Gene regulatory network construction based on the genes with cardiac-specific regulatory elements. Genes with unknown function in the heart were annotated by default with a gray fill and blue border. Other genes (colored fill) with a known function obtained from GO annotation through PANTHER [35] are placed in categories of “Angiogenesis” of “blood” (red), “Heart development (pink),” “Muscle development” (green), “Cell cycle” (purple), “Ion channel activity” (blue), and “cytoskeleton” (yellow). Red border indicates association with known heart defects (data from Mouse Phenotype Database [36]). Double-line border indicates annotated expression in the heart from the GXD database [32]. A Network of genes with interacting partners from STRING database [19]. B Genes with no interacting partners in the STRING database at the time of this study