Fig. 5

Increased compaction of inactive X chromosomes in comparison to active X chromosomes is present at the TAD-to-chromosome scale, but not at the TAD/sub-TAD scale. A Radii of gyration of inactive (left, N = 403) versus active X chromosomes (right, N = 421) from fine-scale chromatin tracing of the 840-kb region (target region of A as stated in Fig. 1). B Radii of gyration of inactive (left, N = 95) versus active X chromosomes (right, N = 95) from large-scale chromatin tracing of 40 TADs across the entire X chromosome. C Schematic illustration of non-proportional compaction of inactive X chromosomes at length scales above the TADs/single-cell domains. The p values were calculated for radii of gyration using two-sided Student’s t tests. Boxes are defined as in Fig. 3G