Fig. 5

QKI5 preferentially binds to activated gene promoters. a Percentages of different genic and intergenic regions associated with QKI5 peaks as identified by ChIP-seq in THP-1 cells (QKI5-bound). The abundance of each type of region in the human genome (Genome) is shown for comparison. The numbers in the parentheses indicate the enrichment ratio relative to the genome; P values were evaluated by single-tailed Fisher’s exact test (*P value < 0.05). b Metaplot showing the distribution of H3K4me3 and H3K27ac ChIP-seq (left panel) and Pol II ChIP-seq fragment depth (right panel) within − 3000 bp to 3000 bp centered around QKI5 ChIP-seq peaks on promoter regions. c Genomic visualization of QKI5, H3K4me3, and Pol II ChIP-seq datasets on the indicated gene loci. d QKI5 ChIP-seq signal validation by ChIP-qPCR on the target gene loci in Ctrl- or QKI5/QKI5 M1-overexpressing cells (upper panel), as well as in shCtrl- or shQKI5-treated THP-1 cells (lower panel). Shown is the mean DNA enrichment relative to input; error bars indicate standard deviations around means from three biological replicates. Asterisks indicate significant differences between the specified samples (*P value < 0.05, **P value < 0.01, *** P value < 0.001, t test). e Venn diagram illustrating the intersection of QKI5-bound genes and QKI5/QKI5 M1/PMA commonly activated genes, P value was evaluated by double-tailed Fisher’s exact test (*P value < 0.05). f Scatterplot shows log2 (fold change) of expression level of overlapping DEGs obtained from QKI5 and QKI5 M1-overexpressing cells, then filtered by the presence of QKI5 ChIP peaks. The purple dots indicate selected target genes