Fig. 4

Comparing PAS site identification and PAU quantification across methods. a Barplot showing the proportion of Iso-seq PAS sites across 2862 genes that were identified by 3'-Seq and short-read RNA-seq methods. These methods are able to identify, at best, ~75% PAS sites identified by Iso-Seq. b Comparison of PAU calls across methods. Error(Sum |Delta PAU|) refers to the concordance in calls between two methods, A and B (as outlined in the schematic). The solid and dotted lines represent comparison of all methods against Iso-Seq, and 3′-Seq, respectively. c Receiver operating characteristic (ROC) curves. True positives are instances in which Iso-Seq with PAUs > 5% have analogous PAS sites defined by other methods with PAUs > 0%. False positives are PAS sites defined by other methods with PAUs > 5% that lack analogous PAS sites defined by Iso-Seq. d H3F3B PAS sites and (PAUs ≥ 1%)