Fig. 1
Induction of KrasG12D leads to changes in cell features. a iKras cells (4292, 9805, and 1012) were cultured without doxycycline (0 hr) or treated with doxycycline (48 hrs) to induce KrasG12D. Proliferation was measured by cell confluence in all four lines and expressed as a ratio of KrasG12D-expressing cells to control for each cell line. Cell confluency is an average of three separate experiments for each line. b Western blot analysis was performed in 4292 iKras cell line at 0, 12, 24, and 48 hrs time points. Cell lysates were probed with the indicated antibodies for KRASG12D and the histone marks. c Densitometry of western blots was performed on three separate experiments. * and ** indicates p value < 0.05 and 0.01 respectively. All data is expressed as mean ± SEM