Fig. 6

H3K27me3 is decreased on Polycomb target sites in genes and transposable elements (TEs) by depletion of MpBMI1/1L. a, b Boxplots showing H2Aub levels (RPKM, reads per kilobase per million mapped reads) on only-H2Aub genes (a) and H2Aub/H3K27me3 genes (b) in WT and Mpbmi1/1l mutants. c, d Boxplots showing H3K27me3 levels on H2Aub/H3K27me3 genes (c) and only-H3K27me3 genes (d) in WT and Mpbmi1/1l mutants. e Boxplot showing H3K27me3 levels of genes with reduced H2Aub level in Mpbmi1/1l mutants. H2Aub and H3K27me3 levels were calculated as the average RPKM from 1 kb upstream of the transcriptional start to the transcriptional end of genes. f GO terms of genes with reduced H2Aub level in Mpbmi1/1l mutants. Response pathways are marked in red. g, h Boxplots showing H2Aub (g) and H3K27me3 levels (h) on H2Aub/H3K27me3 TEs in wild type (WT) and Mpbmi1/1l mutants. H2Aub and H3K27me3 levels were calculated as the average RPKM from the start to the end of TEs. Boxes show medians and the interquartile range, and error bars show the full range excluding outliers. n.s., not significant; **p < 0.01 (Wilcoxon test). i Genome browser views of two selected H2Aub/H3K27me3 TE loci showing decreased H2Aub and H3K27me3 marks in Mpbmi1/1l mutants compared to WT