Fig. 5

H2Aub is essential for PRC1-mediated transposable element activation. a, b Enriched GO terms of commonly upregulated genes in overlap 1 (panel a in Fig. 4) and overlap 2 (panel a in Fig. 4). Response pathways are marked in red. c, d Enriched GO terms of commonly downregulated genes in overlap 3 (panel b in Fig. 4) and overlap 4 (panel b in Fig. 4). Response pathways are marked in red. e Venn diagram showing overlap of downregulated transposable elements (TEs) in Mph2a;H2AK115R/K116R, Mph2a;H2AK119R, and Mpbmi1/1l mutants. Significance was tested using a Hypergeometric test. f Boxplot showing expression level (RPKM, reads per kilobase per million mapped reads) of TEs in overlap 5 (panel e) in WT, Mph2a;H2AK115R/K116R, and Mpbmi1/1l mutants. g Boxplot showing expression level (RPKM) of TEs in overlap 6 (panel e) in WT, Mph2a;H2AK119R, and Mpbmi1/1l mutants. h, i Boxplots showing H2Aub levels of TEs in the group of overlap 5 (h) and overlap 6 (i). H2Aub levels were calculated as the average RPKM from the start to the end of TEs. Boxes show medians and the interquartile range, and error bars show the full range excluding outliers. **p < 0.01 (Wilcoxon test)