Fig. 8

Joint-specific enhancers and chromatin interactions in the RBPJ locus might influence the joint-specific expression of RBPJ. a CAGE measurements of active enhancers (pink bars) and active promoters (light blue bars) in FLS from knees (n = 2), metacarpophalangeal (MCP) joints (n = 3), and shoulders (n = 2). Red box highlights enhancer used in MCP joints overlapping risk SNPs. Green box highlights main enhancer in shoulders. b Representative depiction of open chromatin as measured by ATAC-seq (black bars) in FLS from knees (n = 3), MCP joints (n = 2), and shoulders (n = 2) and representative depiction of ChromHMM regulatory regions in FLS from knees (n = 3), MCP joints (n = 2), and shoulders (n = 2). Green box highlights main enhancer in shoulders. c Chromatin interactions in FLS from knees (n = 2), MCP joints (n = 2), and shoulders (n = 2) as measured by CHiC. Green box highlights interaction of the shoulder enhancer with the risk locus