Fig. 5

Short and long-term 1,6-HD treatment respectively switched the intermediate compartments to A and B types. A Top: average size of compartment A regions. Bottom: total length of compartment A regions. All p values were determined by the Wilcoxon rank-sum test. B Top: average size of compartment B regions. Bottom: total length of compartment B regions. All p values were determined by the Wilcoxon rank-sum test. C Proportion of constant A, constant B, switch and filtered regions (with low mapping ration or high copy number). The blown box showing the details of switch regions. “Early-affected” refers to switch regions in 1/2/5 min while “Late-affected” refers to switch regions in 10/30 min. “Recoverable” refers to switch regions have same state in withdrawal and 0 min while “Irrecoverable” refers to switch regions have different states in withdrawal and 0 min. D Venn plot of switch regions (purple) and regions with low PC1 value (− 0.5 < PC1 < 0.5) (grey). The p value was determined by hypergeometric test. E Density plot of the PC1 distribution of constant regions with low PC1 value at each time point. Black dashed line indicates the median value. Background color indicates compartment assignments (A, red; B, blue). F Density plot of the PC1 distribution of switch regions with low PC1 value at each time point. Black dashed line indicates the median value. Background color indicates compartment assignments (A, red; B, blue). G Density plot of size distribution of constant regions and switch regions with low PC1 value. Black dashed line indicates the median value. The p value was determined by the Wilcoxon rank-sum test. H Fold enrichment (median signal within interested regions / median signal across the whole genome) of ChIP-seq signal of condensate components. All p values were determined by the Wilcoxon rank-sum test. I Example (chr1: 34.2–47.7 Mb) showing constant regions and switch regions with low PC1 value. Each track: PC1 indicates compartment assignments at each time point (A, red; B, blue). J Schematic indicating that short- (2 min) and long-term (30 min) 1,6-HD treatment respectively switched the intermediate compartments to A and B types