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Table 2 Comparison of sequencing methods

From: Oncogene Concatenated Enriched Amplicon Nanopore Sequencing for rapid, accurate, and affordable somatic mutation detection

 

OCEANS

Standard NGS

NGS w/ UMI

 

(MinION)

(MiSeq)

(MiSeq)

Samples / flow cell

24

24

2

Flow cell price

<DOLLAR/>900

<DOLLAR/>1300

<DOLLAR/>1300

Flow cell reusability

10

1

1

Library preparation kits price

<DOLLAR/>890

<DOLLAR/>750

<DOLLAR/>63

Sequencing price / sample

<DOLLAR/>7.45

<DOLLAR/>85.4

<DOLLAR/>682

Turnaround time

10 h

24-72 h

24–72 h

Single-base VAF LoD

0.05–1%

2–5%

0.1–0.5%